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| Registros recuperados: 82 | |
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| Gueguen, Yannick; Romestand, Bernard; Fievet, Julie; Schmitt, Paulina; Destoumieux Garzon, Delphine; Franck, Vandenbulcke; Philippe, Bulet; Bachere, Evelyne. |
| A cDNA sequence that encodes a 61-amino acid polypeptide precursor with homologies to proline-rich antimicrobial peptides (AMPs) was identified in the oyster Crassostrea gigas. After release of a hydrophobic signal peptide. the resulting 37-amino acid peptide, Cg-Prp, is composed of an acidic region and a cationic proline-rich region. To evaluate the biological properties of Cg-Prp, multiple proline-rich peptides corresponding to putative processing of the full-length Cg-Prp were synthesized. A limited antimicrobial activity was observed for two of them, which also showed strong synergistic antimicrobial activity with Cg-Def, a defensin from C gigas. To our knowledge, this is the first evidence of synergy between a defensin and another AMP in an... |
| Tipo: Text |
Palavras-chave: Antimicrobial peptide; Mollusk; Synergy; Invertebrate; Innate immunity; Pacific oyster. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6232.pdf |
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| Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
| Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects... |
| Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf |
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| Gagnaire, Beatrice; Thomas Guyon, H; Burgeot, Thierry; Renault, Tristan. |
| The shellfish industry is an important economic activity in France, occurring mostly in estuarine zones subject to pollution due to anthropogenic activities. The harmful effects of pollutants on species inhabiting these estuarine zones are not well known. Among marine species, bivalve mollusks---particularly Pacific oyster, Crassostrea gigas-may serve a model of interest. The species is sedentary and filter-feeding, which favors bioaccumulation of pollutants in their tissues. Oysters may be suitable for studies on disturbance by pollutants of physiological activities, among which defense mechanisms are poorly documented in bivalves. In this study, effects of pollutants on hemocyte functions were monitored in Pacific oyster, C. gigas. Hemocytes were exposed... |
| Tipo: Text |
Palavras-chave: Toxicity; Pesticides; PCBs; PAHs; Crassostrea gigas; Pacific oyster; Hemocytes; Flow cytometry; Cellular activities. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1695.pdf |
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| Samain, Jean-francois; Ropert, Michel; Bedier, Edouard; Soletchnik, Patrick; Mazurie, Joseph; Le Coz, Florence; Blin, Jean-louis; Mille, Dominique; Pajot, Régis; Haffray, Patrick; Bacher, Cedric; Grangere, Karine; Grangere, Karine; Pouvreau, Stephane; Bourles, Yves; Gohin, Francis; Woerther, Patrice; Boudry, Pierre. |
| Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in Japan, US and Europe. It affects both adults and juveniles and the causes of this phenomenon are complex. Environmental, physiological or pathological putative causes were studied all over the world. Recently, the MOREST project ((MORtalitEStivale) a multidisciplinary project (2001-2005) focused on his problem led to a better understanding of this phenomenon in France. The results showed in these geographical conditions, the main interactions between the oysters, their environment and certain pathogens. |
| Tipo: Text |
Palavras-chave: Interaction model; Environmental parameters; Summer mortality; Crassostrea gigas; Pacific oyster. |
| Ano: 2006 |
URL: https://archimer.ifremer.fr/doc/2006/acte-3476.pdf |
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| Sauvage, Christopher; Boudry, Pierre; De Koning, D. -j.; Haley, C. S.; Heurtebise, Serge; Lapegue, Sylvie. |
| P>Summer mortality is a phenomenon severely affecting the aquaculture production of the Pacific oyster (Crassostrea gigas). Although its causal factors are complex, resistance to mortality has been described as a highly heritable trait, and several pathogens including the virus Ostreid Herpes virus type 1 (OsHV-1) have been associated with this phenomenon. A QTL analysis for survival of summer mortality and OsHV-1 load, estimated using real-time PCR, was performed using five F-2 full-sib families resulting from a divergent selection experiment for resistance to summer mortality. A consensus linkage map was built using 29 SNPs and 51 microsatellite markers. Five significant QTL were identified and assigned to linkage groups V, VI, VII and IX. Analysis of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Herpes virus; Pacific oyster; QTL mapping; Summer mortality. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12241/9063.pdf |
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| Petton, Bruno. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient une fréquence plus faible de portage latent. Dans ce contexte de crise zoo-sanitaire chronique, l’objectif de notre étude consistait à obtenir une information du statut zoo-sanitaire OsHV-1 μVar des populations de... |
| Tipo: Text |
Palavras-chave: Huitre creuse; Naissain; Survie; OsHV-1 μ Var; Epreuve thermique.; Pacific oyster; Spat; Survival; OsHV-1 μVar; Thermal challenge. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00335/44586/44304.pdf |
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| Petton, Bruno; Riobe, Marion; Koechlin, Hugo; Queau, Isabelle; Mingant, Christian; Ratiskol, Dominique; Le Grand, Jacqueline; Petton, Sebastien; Thomas, Yoann; Alunno-bruscia, Marianne; Pouvreau, Stephane; Pernet, Fabrice; Maurer, Daniele; D'Amico, Florence; Lagarde, Franck; Mortreux, Serge; Roque D'Orbcastel, Emmanuelle; Mille, Dominique; Geay, A.; Bouquet, A.-l.; Cochet, Helene. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient un moindre risque de contamination. Dans le contexte de crise sanitaire chronique, l’objectif de notre étude était d’ obtenir une information qualitative relative au statut zoo-sanitaire OsHV -1 μ V ar des... |
| Tipo: Text |
Palavras-chave: Huitre creuse; OsHV - 1μVar; Epreuve thermique.; Maladie; Naissain; Pacific oyster; Spat; Disease; Thermal challenge; OsHV-1 μVar. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38682/37180.pdf |
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| Petton, Bruno; Le Grand, Jacqueline; Riobe, Marion; Mingant, Christian; Ratiskol, Dominique; Petton, Sebastien; Alunno-bruscia, Marianne. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Depuis 2010, des travaux de qualification zoo-sanitaire ont montré qu’environ un lot de naissains de captage sur deux était infecté par le virus avant l’apparition des mortalités dans le milieu naturel. En 2014, dans le cadre de la première action QUALIF, les résultats de la qualification zoo-sanitaire des naissains du captage de l’année 2013 à l’échelle nationale ont mis en évidence le caractère infectieux des maladies développées par ces naissains porteurs latents d’OsHV-1 μVar. L’objectif 2015 du... |
| Tipo: Text |
Palavras-chave: Huitre creuse; Naissain; Maladie; OsHV-1 μVar; Epreuve thermique.; Pacific oyster; Seed; Disease; OsHV-1 μVar; Thermal challenge.. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00335/44587/44305.pdf |
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| Roberts, S; Gueguen, Yannick; De Lorgeril, Julien; Goetz, F. |
| Interleukin-17 (IL-17) gene models have been found in the sequenced genomes of Strongylocentrotus purpuratus and Caenorhabditis elegans. However, there have been no published reports on the empirical cloning and characterization of any interleukin cDNAs in invertebrates. From a Pacific oyster (Crossostrea gigas) hemocyte cDNA library, two clones were obtained that encoded a protein similar to vertebrate IL-17s. The putative oyster IL-17 homolog (CgIL-17) was 27% identical to rainbow trout IL-17D, 21% to human IL-17D and 24% to an IL-17D-like gene model obtained from the annotation of the sea urchin genome. IL-17s from the oyster, sea urchin, trout and human, contained conserved cysteine residues found in all forms of IL-17 in mammals. Injection of bacteria... |
| Tipo: Text |
Palavras-chave: Invertebrate innate immunity; Crassostrea gigas; Invertebrate cytokines; Hemocytes; Pacific oyster; Interleukin. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4151.pdf |
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| Soletchnik, Patrick; Ropert, Michel; Mazurie, Joseph; Fleury, Pierre-gildas; Le Coz, Florence. |
| Oyster mortality was monitored at multiple sites along the French coastline (around 5 500 km) between 1993 and 2005. Mortality data for one- and two-year-old oysters were collected over 1012 years in 39 oyster culture sites bordering 3 different "seas": the English Channel, Bay of Biscaye and Mediterranean. Combining these data with records from environmental monitoring databases, 11 of these sites had consistent chronological data sets including both environmental data and mortality records. Mortality in one-year-old oysters was clearly summer mortality (49% of their annual mortality) whereas mortality of two-year-olds occurred mostly in spring (51%). Analysis of variance revealed that "coastal area" was the main influence on mortality of one-year-olds... |
| Tipo: Text |
Palavras-chave: Mortality; Environment; Crassostrea gigas; Pacific oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3295.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf |
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| Song, Yun-peng; Suquet, Marc; Queau, Isabelle; Lebrun, Luc. |
| The quality of oyster spermatozoa has become an issue for modern aquaculture. However, reliable protocols used to assess gamete quality are lacking. The aim of this work is to define a standardised experimental fertilisation protocol for Pacific oyster oocytes. Six experiments have been carried out in this study. The optimal conditions for Pacific oyster experimental fertilisation have been defined: (1) oocytes can be conserved in seawater for at least 4 h before fertilisation. (2) oocyte concentration: 100-1000 ml(-1) seawater, (3) fertilisation volume: 10-100 nil, (4) spermatozoa: oocyte ratio: 400 to maintain discriminating conditions, (5) gamete contact time: longer than 10 mill and (6) fertilisation and incubation temperatures: both 19 degrees C. The... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Pacific oyster; Experimental; Fertilisation. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6231.pdf |
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| Registros recuperados: 82 | |
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